Methods Perform acute toxicity test in mice, as well as local stimulation test, systemic anaphylaxis test, long-term toxicity test and skin allergic reaction test in guinea pigs on live Brucella vaccine. Immunize mice with the vaccine and determine the serum antibody. 方法对布氏菌活疫苗进行小白鼠急性毒性试验、局部刺激试验、全身过敏试验、长期毒性试验及豚鼠皮肤变态反应试验并免疫小鼠,进行血清抗体检测。